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Anti-aging & longevity

PNC-27

p53-derived peptide · PNC27

PreclinicalReviewed 18 Aug 2026

PNC-27 is a fragment of p53, the tumour suppressor protein, fused to a sequence that carries it across membranes. The claim behind it is specific and unusual: that it binds HDM-2 on the surface of cancer cells and forms pores, killing them directly, while sparing normal cells that do not display HDM-2 at the membrane.

The laboratory work behind that claim is real. Published cell-culture studies show selective killing of cancer cell lines with normal cells left intact, and that selectivity is what makes it interesting rather than merely cytotoxic.

It has never been tested in a human being. Not a phase 1, not a case series, nothing. And it is sold, at considerable prices, largely to people who have just had a cancer diagnosis. That is why this page is written the way it is. The gap between selective killing in a dish and a treatment is where essentially every cancer drug candidate in history has failed, and the people most drawn to this compound are the ones with least room to absorb that failure.

How it works

The p53-derived segment binds HDM-2, a protein that cancer cells are reported to display on their outer membrane and normal cells largely do not. That differential display is the entire basis of the selectivity claim.

Fused to a membrane-crossing sequence, the bound peptide is proposed to form pores in the membrane, causing the cell to die by leakage rather than by any signalling pathway. That is a physical mechanism, not a regulatory one.

In cell culture this produces selective killing of tumour lines. Whether enough peptide survives in a body, reaches a tumour, and preserves that selectivity among the many cell types in a person is the question no study has approached.

Membrane-disrupting peptides are not gentle molecules. Selectivity that holds in a dish of two cell types is a much weaker claim than selectivity that holds in a person.

Regulatory status — United States

Not approved anywhere, and never studied in a human trial for any indication. Sold as a research chemical. Marketing it as a cancer treatment is not supported by any human evidence.

Last reviewed 18 Aug 2026. Regulatory positions in this category change frequently; we date this line so you can see how current it is.

What it is studied for

Each entry states the evidence behind it. We do not rank indications by effectiveness — for most compounds here, that would be a claim nobody can support.

Cancer

Published review

What it is sold for.

EvidenceSelective killing of cancer cell lines in culture, and some animal work. No human trial of any kind has been conducted. No phase 1, no case series.

Identity and clearance

Molecule

Class
p53-derived peptide fused to a membrane-penetrating sequence
Length
32 amino acids

Dose protocols reported in sources

Displayed for reference only. Every row says where it came from, and a trial protocol and a community convention are not the same kind of information. None of this is fed into the calculator, and none of it is a recommendation.

Dose protocols reported in sources, with the source of each.
GoalDoseFrequencyRouteUnits, 5 mg vialSourceStart this protocol
Commonly described community protocol1 mg – 5 mgDaily, in coursesSubcutaneousThere is no trial behind any figure here, in any species, at any dose. These numbers circulate without a source.20–100 uCommunity practiceStart this

Start this copies a row into a protocol of your own, which you can then edit. Where a source gave a dose range or an ambiguous frequency, those fields arrive empty — we will not pick a number on your behalf.

CheckRows marked community practice describe what people commonly do, drawn from public discussion. They are not derived from any trial and are not evidence that a dose is safe or effective.

Calculator

Work out what to draw

Pre-filled with a 10 mg vial as a worked example. These are not recommended values — change them to match the vial in front of you.

Dose unit
Your syringe
010203040506070809010040 units0.4 mL

Draw to 40 units on a 1 mL U-100 syringe. That is 0.4 mL, containing 2 mg of PNC-27.

Concentration5mg / mL
Volume drawn0.4mL
Doses per vial5doses
Per unit50mcg / unit

40 units sits exactly on a printed line.

Bioalmanac performs arithmetic on values you enter. It does not recommend doses, schedules or compounds, and nothing here is medical advice. Confirm every calculation against your vial and syringe before drawing, and speak with a licensed healthcare provider.

Open this calculation in the full calculator

Units, concentration, bacteriostatic water — the glossary defines the vocabulary, and a unit is not a fixed volume.

Safety

Reported effects

  • EverythingCommunity practice

    Unknown

    No human has ever been studied on this compound. No adverse event has been recorded because nobody has looked.

  • Membrane damage to cells that are not cancerPublished review

    Mechanistically possible, unquantified

    The selectivity claim rests on differential HDM-2 display observed in culture. Whether it holds across every tissue in a body is exactly what has not been tested.

  • Tumour lysis effectsPublished review

    Unknown

    If it killed tumour cells at scale, the metabolic consequences of that would themselves be dangerous and require monitoring nobody self-administering has.

When to stop

  • Any acute reaction: fever, breathlessness, chest pain, collapse. Seek emergency care.
  • Dark urine, reduced urine output, or confusion.
  • Any allergic response: rash, hives, facial swelling or difficulty breathing. Seek urgent care.
  • Any deterioration in a diagnosed condition. Tell your oncology team what you have taken.

Interactions and situations that need care

  • Using it instead of, or alongside, cancer treatmentAvoid

    This is the situation the compound is sold for and the one where it does most harm. There is no human evidence of any kind. Delaying or displacing treatment that has evidence behind it — for one that has none — costs time that in oncology is not recoverable. If you are taking it alongside treatment, your oncology team needs to know. Unlisted compounds interact with chemotherapy and confound the assessment of whether treatment is working.

  • Intravenous self-administrationAvoid

    Injecting an uncharacterised membrane-disrupting peptide directly into the bloodstream, outside a clinical setting, removes every barrier that would otherwise slow a severe reaction.

  • Pregnancy and breastfeedingAvoid

    A compound designed to lyse cells has no version of appropriate use in pregnancy, and no safety data exists.

What to expect

HonestlyThere is no human efficacy data for this compound, so there is no evidence-based timeline to give. Any site publishing a week-by-week schedule of expected effects has invented it.

  • No human has ever been studied on this compound. There is no phase 1 and no published case series.
  • The cell-culture work is real, and selective killing in a dish is where nearly every cancer drug candidate in history has also looked promising.
  • It is sold mainly to people who have just had a diagnosis. That is the context in which an untested compound does the most damage, because the alternative it displaces is time-critical.
  • If you are taking it, tell your oncology team. Not for permission. Because it can interact with treatment, and confuse the reading of whether that treatment is working.

Storage and handling

Freeze-dried powder
Refrigerated at 2–8 °C, protected from light.
After mixing
Refrigerated at 2–8 °C. Do not freeze once mixed.
Long-term, frozen
Unopened powder is stable at −20 °C or colder.

Printable one-pagerHow to reconstitute a vial, step by step

Checking your vial

Research chemicals carry no manufacturing standard, so what the vial looks like is often the only quality signal available before you use it. More on assessing quality and COAs.

  • White cake, clear solution

    Standard for a lyophilised peptide.

  • Collapsed cake or cloudy solution

    Heat damage or degradation. Do not use it.

Questions

Does it really kill only cancer cells?
In cell culture it selectively killed tumour lines while sparing normal cells, which is a genuine published finding. Whether that selectivity survives contact with a whole body — with its many cell types — its circulation and its immune response, has never been tested in anything approaching a human trial.
Has anyone taken it?
People buy and use it. What does not exist is any study recording what happened to them. Anecdote from vendors and forums is not the same as a case series, and it systematically under-reports the outcomes nobody wants to post about.

References

  1. PNC-27: HDM-2 binding and selective membrane lysis of cancer cell lines

    Cancer cell biology literature, 2010 · Cell culture studies with some animal work

    In vitro, with rodent models

    Selective killing of cancer cell lines attributed to binding membrane-displayed HDM-2 and forming pores, with normal cells reported largely unaffected. No human trials of any kind have been conducted.

  2. Commonly reported community protocols

    Bioalmanac editorial summary of public community sources, 2026 · Not a study

    Records dose and route patterns described in public discussion. Carries no evidential weight about safety or effect.

Keep this page honest

If something here is wrong, out of date, or missing a source, tell us and we will fix it and say that we did.

Suggest a correction

This page is educational. It describes what published research reports, not what you should do. Peptides discussed here are largely not approved for human use, and nothing on Bioalmanac is medical advice. No clinician reviews these pages.

Page last updated 18 Aug 2026